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For the purpose of utilizing cellulose resources by cellulolytic enzymes of Stropharia rugosoannulata, it´s cultural conditions for the prodution of cellulolytic enzymes in synthetic media were investigated. The optimum pH for the production of Avicelase and β-glucosidase was pH 5.0, while that of CMCase was pH 4.0. The optimum temperature for the production of Avicelase, CMCase and β-glucosidase was 40℃. Among the carbon sources, xylose was good for the production of CMCase and β-glucosidase, but maltose was good for the production of Avicelase. The optimum concentration of the carbon sources for the production of CMCase, Avicelase and β-glucosidase was 1.0, 0.8 and 1.1 %, respectively. As inorganic nitrogen sources, NH₄Cl was good for the production of all the three cellulolytic enzymes. The optimum concentration of NH₄Cl for the production of CMCase was 0.3% while that of Avicelase and β-glucosidase was 0.4%. As organic nitrogen sources, malt extract was good for the production of all the three cellulolytic enzymes. The optimum concentration of organic nitrogen for the production of β-glucosidase was 1.3% while that of CMCase and Avicelase was 1.0%. As the mineral sources, CoCl₂ good for the was good for the production of all the three cellulolytic enzymes. The optimum concentration of CoCl₂ for the production of all the three enzymes was 0.35%.
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